Multitailed T2 bacteriophage

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Preservation of T2 Bacteriophage with Liquid Nitrogen.

There have been few reports on the preservation of viruses at the temperature of liquid nitrogen (-196 C). In this study, factors affecting the survival of phage were observed. Phage lysates in broth did not lose titer after a storage period of 4 weeks in liquid nitrogen. The rate of freezing was not critical, but freezing in nitrogen vapor was not satisfactory. When the virus was partially pur...

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A specific reversible inhibition of bacteriophage T2.

With most phages, the titer of a lysate measured by plaque count remains stable from the time of lysis or shows a progressive decrease as phage becomes inactivated by a variety of environmental agents. Freshly prepared lysates of bacteriophage T2 of Escherichia coli rise in titer spontaneously with time, the maxmum titer being reached in several weeks under refrigeration. Bertani (unpublished d...

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The structural unity of the DNA of T2 bacteriophage.

The molecular weight of the DNA extracted from the phages T2 and T4 has been the subject of several investigations in recent years. Autoradiographic studies (Levinthall and Levinthal and Thomas2) of P32-labeled DNA showed that the material after embedding in photographic emulsions consisted on the average of one large piece of DNA per phage particle plus smaller material. The large piece had a ...

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Isologous Interference with Ultraviolet and X-ray Irradiated Bacteriophage T2.

Levy, Stuart B. (Institut du Radium, Paris, France). Isologous interference with ultraviolet and X-ray irradiated bacteriophage T(2). J. Bacteriol. 87:1330-1338. 1964.-Qualitative and quantitative analysis of the interference capacity of an irradiated T(2) bacteriophage was made with ultraviolet and X-ray irradiation. Two different effects were found to explain the total interference picture in...

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Decay of Incorporated Radioactive Phosphorus during Reproduction of Bacteriophage T2

The multiplication of vegetative T2 bacteriophage in B/r bacteria has been followed by studying the lethal effects of decay of incorporated radiophosphorus P(32) at various stages of the eclipse period. Experiment I. Non-radioactive B/r bacteria were infected with highly radioactive (i.e. P(32)-unstable) T2 and infection allowed to proceed at 37 degrees C. for various numbers of minutes before ...

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ژورنال

عنوان ژورنال: Journal of Virology

سال: 1967

ISSN: 0022-538X,1098-5514

DOI: 10.1128/jvi.1.5.1087-1088.1967